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Molecular Enzymology
London Research Institute -Clare Hall Laboratories- -Cancer Research UK- Charity Registration No. 1089464 -Terms and Conditions- |
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| Sequence of DNA ligase from B. st. |
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ID BST011676 standard; DNA; PRO; 2013 BP. AC AJ011676; NI e1329584 DT 01-OCT-1998 (Rel. 57, Created) DT 01-OCT-1998 (Rel. 57, Last updated, Version 1) DE Bacillus stearothermophilus lig gene KW DNA ligase; lig gene. OS Bacillus stearothermophilus OC Bacteria; Firmicutes; Bacillus/Clostridium group; Bacillaceae; Bacillus. RN [1] RP 1-2013 RA Brannigan J.A.; RL Submitted (29-SEP-1998) to the EMBL/GenBank/DDBJ databases. RL Brannigan J.A., Chemistry, University of York, Heslington, York, YO10 RL 5DD, UK. RA Brannigan J.A., Ashford S.R., Doherty A.J., Timson D.J., Wigley D.B.; RT "Cloning and expression of DNA ligase from Bacillus stearothermophilus"; RL Unpublished. DR SPTREMBL; O87703; O87703. FH Key Location/Qualifiers FT source 1..2013 FT /organism="Bacillus stearothermophilus" FT /strain="NCA1503" FT CDS 1..2013 FT /db_xref="PID:e1329585" FT /db_xref="SPTREMBL:O87703" FT /evidence=EXPERIMENTAL FT /gene="lig" FT /product="DNA ligase" FT /EC_number="6.5.1.2" FT /translation="MDRQQAERRAAELRELLNRYGYEYYVLDRPSVPDAEYDRLMQELI FT AIEEQYPELKTSDSPTQRIGGPPLEAFRKVAHRVPMMSLANAFGEGDLRDFDRRVRQEV FT GEAAYVCELKIDGLAVSVRYEDGYFVQGATRGDGTTGEDITENLKTIRSLPLRLKEPVS FT LEARGEAFMPKASFLRLNEERKARGEELFANPRNAAAGSLRQLDPKVAASRQLDLFVYG FT LADAEALGIASHSEALDYLQALGFKVNPERRRCANIDEVIAFVSEWHDKRPQLPYEIDG FT IVIKVDSFAQQRALGATAKSPRWAIAYKFPAEEVVTTLIGIEVNVGRTGVVTPTAILEP FT VRVAGTTVQRATLHNEDFIREKDIRIGDAVIIKKAGDIIPEVVGVVVDRRDGDETPFAM FT PTHCPECESELVRLEGEVALRCLNPNCPAQLRERLIHFASRAAMNIEGLGEKVVTQLFN FT AGLVRDVADLYCLTKEQLVGLERMGEKSAANLLAAIEASKQNSLERLLFGLGIRYVGAK FT AAQLLAEHFETMERLERATKEELMAVPEIGEKMADAITAFFAQPEATELLQELRAYGVN FT MAYKGPKRSAEAPADSAFAGKTVVLTGKLASMSRNEAKEQIERLGGRVTGSVSRSTDLV FT IAGEDAGSKLEKAQQLGIEIWDESRFLQEINRGKR"
SQ Sequence 2013 BP; 435 A; 549 C; 651 G; 378 T; 0 other; atggaccgcc aacaagccga acggcgcgcg gccgagctgc gcgaactgtt gaaccgctac 60 gggtacgaat attatgtgct tgaccgcccg tctgttccag atgccgagta tgaccggttg 120 atgcaagagc tcatcgccat tgaggaacaa tatccggagc tcaaaacgag cgattcgccg 180 acccagcgca tcggcggtcc gccccttgag gcgtttcgca aagtggcgca tcgcgttccg 240 atgatgagct tggcgaacgc gtttggcgaa ggagacttgc gcgattttga ccgccgcgtc 300 cgtcaggaag tcggggaagc ggcgtacgta tgcgagctga aaatcgacgg cctcgccgtc 360 tcggtccgct atgaagacgg ctatttcgtc caaggggcga cgcgcggcga cgggacgacg 420 ggtgaagaca ttacggaaaa tttgaaaacg atccgctcgc tgcctctccg tctgaaagaa 480 ccggtgtcgc ttgaagcgcg cggcgaggcg tttatgccga aagcgtcgtt tttgcgcttg 540 aatgaggagc gaaaagcccg cggcgaggag ctgtttgcca acccgcgcaa cgccgcggct 600 ggatcgctcc gccaactcga cccgaaagtg gcggcctcgc gccagctgga tttgtttgta 660 tatggcttag ccgacgccga ggcgctcggc atcgcttcac acagcgaagc gcttgattat 720 ttgcaggcgc tcgggtttaa agtaaaccca gagcgacggc gctgcgccaa tattgatgag 780 gtgatcgcct ttgtcagcga gtggcacgac aagcggccgc agctgccgta tgagattgac 840 ggcatcgtta ttaaggtgga ctcattcgcc cagcaacggg cgcttggcgc gacggcgaaa 900 agcccgcgct gggcgattgc ctacaagttc ccagccgagg aagtggtgac gacgttaatc 960 ggcattgagg tgaacgtcgg ccgcacgggt gtcgtcacgc cgacggcgat tttagagccg 1020 gtgcgcgtcg ccggcacgac ggtgcagcgc gccacccttc acaatgaaga ttttatacgg 1080 gaaaaagaca ttcgcatcgg cgatgcggtc atcattaaaa aagcgggcga catcatcccg 1140 gaagtggtcg gcgtcgtcgt tgaccggcgc gatggggatg aaacgccgtt tgcgatgccg 1200 acgcactgcc cggaatgcga aagcgagctc gtccgtctcg agggggaagt ggcgctccgc 1260 tgcttgaacc cgaactgccc ggcccagctg cgcgagcggc tcatccattt tgcctcaagg 1320 gcggcgatga acatcgaggg tttgggcgaa aaagtcgtca cccaactgtt taacgccggc 1380 cttgtccgcg atgtcgctga tttgtactgc ctgacgaagg aacagcttgt cgggttggaa 1440 cggatggggg aaaagtcggc ggccaacttg cttgcggcga ttgaggcgtc gaagcaaaac 1500 tcgcttgaac ggctgctgtt tggccttggc attcgctacg tcggagcgaa agccgcccaa 1560 cttttggctg agcattttga aacgatggag cggctcgaac gggcgacgaa agaggagttg 1620 atggccgtcc ccgaaatcgg agagaaaatg gcggatgcga tcacggcgtt cttcgcccaa 1680 cccgaagcaa ctgagctgct ccaggagctg cgcgcttacg gtgtcaatat ggcttacaaa 1740 gggccgaagc ggtcggctga agcgcctgct gactctgcct tcgccggcaa aacggtcgtc 1800 ttgaccggca agctcgcgtc gatgtcgcgc aatgaagcaa aagaacaaat tgagcggctt 1860 ggcgggcgcg tcaccggcag cgtcagccgc agcaccgacc ttgtcatcgc cggcgaggat 1920 gccggatcga agctggagaa agcccagcag cttggcattg agatttggga cgaatcacga 1980 tttttgcaag agatcaacag ggggaaacga tga 2013
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